Efficient Production of Biallelic RAG1 Knockout Mouse Embryonic Stem Cell Using CRISPR/Cas9

BACKGROUND: Recombination Activating Genes (RAG) mutated embryonic stem cells are (ES) cells which are unable to perform V (D) J recombination. These cells can be used for generation of immunodeficient mouse. Creating biallelic mutations by CRISPR/Cas9 genome editing has emerged as a powerful techni...

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Detalles Bibliográficos
Autores principales: Mehravar, Maryam, Shirazi, Abolfazl, Mehrazar, Mohammad Mehdi, Nazari, Mahboobeh, Banan, Mehdi, Salimi, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697863/
https://www.ncbi.nlm.nih.gov/pubmed/31457047
http://dx.doi.org/10.21859/ijb.2205

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697863/
https://www.ncbi.nlm.nih.gov/pubmed/31457047
http://dx.doi.org/10.21859/ijb.2205

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