Engineering a Polyspecific Pyrrolysyl-tRNA Synthetase by a High Throughput FACS Screen

The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA(Pyl) are extensively used to add non-canonical amino acids (ncAAs) to the genetic code of bacterial and eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process to generate an appropriate PylRS/tRNA(Pyl) p...

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Detalles Bibliográficos
Autores principales: Hohl, Adrian, Karan, Ram, Akal, Anastassja, Renn, Dominik, Liu, Xuechao, Ghorpade, Seema, Groll, Michael, Rueping, Magnus, Eppinger, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700097/
https://www.ncbi.nlm.nih.gov/pubmed/31427620
http://dx.doi.org/10.1038/s41598-019-48357-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700097/
https://www.ncbi.nlm.nih.gov/pubmed/31427620
http://dx.doi.org/10.1038/s41598-019-48357-0

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