Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array

We report a general strategy based on digital counting principle that enables an efficient acquisition of enzyme mutants with desired activities from just a few clones within a day. We prepared a high-density femtoliter droplet array, consisting of 1 million uniform droplets per 1 cm(2) to carry out...

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Detalles Bibliográficos
Autores principales: Zhang, Yi, Minagawa, Yoshihiro, Kizoe, Hiroto, Miyazaki, Kentaro, Iino, Ryota, Ueno, Hiroshi, Tabata, Kazuhito V., Shimane, Yasuhiro, Noji, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703874/
https://www.ncbi.nlm.nih.gov/pubmed/31457078
http://dx.doi.org/10.1126/sciadv.aav8185

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703874/
https://www.ncbi.nlm.nih.gov/pubmed/31457078
http://dx.doi.org/10.1126/sciadv.aav8185

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