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CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris
BACKGROUND: Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia. Recently, it has been shown to be a potentially good chassis host for the production of high-value pharmaceuticals and che...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704636/ https://www.ncbi.nlm.nih.gov/pubmed/31434578 http://dx.doi.org/10.1186/s12934-019-1194-x |