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IL-6–HaloTag(®) enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptors

The assignment of cognate odorant/agonist pairs is a prerequisite for an understanding of odorant coding at the receptor level. However, the identification of new ligands for odorant receptors (ORs) in cell-based assays has been challenging, due to their individual and rather sub-optimal plasma memb...

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Detalles Bibliográficos
Autores principales: Noe, Franziska, Frey, Tim, Fiedler, Julia, Geithe, Christiane, Nowak, Bettina, Krautwurst, Dietmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706138/
https://www.ncbi.nlm.nih.gov/pubmed/31453235
http://dx.doi.org/10.14440/jbm.2017.206