Cargando…
IL-6–HaloTag(®) enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptors
The assignment of cognate odorant/agonist pairs is a prerequisite for an understanding of odorant coding at the receptor level. However, the identification of new ligands for odorant receptors (ORs) in cell-based assays has been challenging, due to their individual and rather sub-optimal plasma memb...
Autores principales: | Noe, Franziska, Frey, Tim, Fiedler, Julia, Geithe, Christiane, Nowak, Bettina, Krautwurst, Dietmar |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Journal of Biological Methods
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706138/ https://www.ncbi.nlm.nih.gov/pubmed/31453235 http://dx.doi.org/10.14440/jbm.2017.206 |
Ejemplares similares
-
A bi-functional IL-6-HaloTag(®) as a tool to measure the cell-surface expression of recombinant odorant receptors and to facilitate their activity quantification
por: Noe, Franziska, et al.
Publicado: (2017) -
Recent biomedical advances enabled by HaloTag technology
por: CHEN, Weiyu, et al.
Publicado: (2022) -
Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology
por: Martincová, Eva, et al.
Publicado: (2012) -
HaloTag, a Platform Technology for Protein Analysis
por: Urh, Marjeta, et al.
Publicado: (2012) -
HaloTag-based conjugation of proteins to barcoding-oligonucleotides
por: Yazaki, Junshi, et al.
Publicado: (2020)