Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing

The CRISPR/Cas9 technology has greatly improved genome editing in Saccharomyces cerevisiae over recent years. However, several current CRISPR/Cas9 systems suffer from work-intensive cloning procedures and/or the requirement of co-transforming target cells with multiple system components simultaneous...

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Detalles Bibliográficos
Autores principales: Degreif, Daniel, Kremenovic, Milana, Geiger, Thomas, Bertl, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706142/
https://www.ncbi.nlm.nih.gov/pubmed/31453248
http://dx.doi.org/10.14440/jbm.2018.254

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706142/
https://www.ncbi.nlm.nih.gov/pubmed/31453248
http://dx.doi.org/10.14440/jbm.2018.254

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