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Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing
The CRISPR/Cas9 technology has greatly improved genome editing in Saccharomyces cerevisiae over recent years. However, several current CRISPR/Cas9 systems suffer from work-intensive cloning procedures and/or the requirement of co-transforming target cells with multiple system components simultaneous...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Journal of Biological Methods
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706142/ https://www.ncbi.nlm.nih.gov/pubmed/31453248 http://dx.doi.org/10.14440/jbm.2018.254 |