Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain

The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (SpyCas9), along with a programmable single-guide RNA (sgRNA), has been exploited as a significant genome-editing tool. Despite the recent advances in determining the SpyCas9 structures and DNA cleavage mechanism, the cleavage-compe...

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Detalles Bibliográficos
Autores principales: Zuo, Zhicheng, Zolekar, Ashwini, Babu, Kesavan, Lin, Victor JT, Hayatshahi, Hamed S, Rajan, Rakhi, Wang, Yu-Chieh, Liu, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706240/
https://www.ncbi.nlm.nih.gov/pubmed/31361218
http://dx.doi.org/10.7554/eLife.46500

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706240/
https://www.ncbi.nlm.nih.gov/pubmed/31361218
http://dx.doi.org/10.7554/eLife.46500

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