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β-mercaptoethanol assists efficient construction of sperm bacterial artificial chromosome library
Bacterial artificial chromosome (BAC) library plays a critical role in the strategic research in genomics. Sperm is known as a good source for BAC library construction. However, preparation of intact DNA from the highly condensed sperm nuclei is not easy. Here we developed and validated an efficient...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
jbm
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6708924/ https://www.ncbi.nlm.nih.gov/pubmed/31453223 http://dx.doi.org/10.14440/jbm.2017.167 |