Single-Cell Quantification of mRNA Expression in The Human Brain

RNA analysis at the cellular resolution in the human brain is challenging. Here, we describe an optimised approach for detecting single RNA transcripts in a cell-type specific manner in frozen human brain tissue using multiplexed fluorescent RNAscope probes. We developed a new robust analytical appr...

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Detalles Bibliográficos
Autores principales: Jolly, Sarah, Lang, Verena, Koelzer, Viktor Hendrik, Sala Frigerio, Carlo, Magno, Lorenza, Salinas, Patricia C., Whiting, Paul, Palomer, Ernest
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710275/
https://www.ncbi.nlm.nih.gov/pubmed/31451701
http://dx.doi.org/10.1038/s41598-019-48787-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710275/
https://www.ncbi.nlm.nih.gov/pubmed/31451701
http://dx.doi.org/10.1038/s41598-019-48787-w

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