Conformational Dynamics and Cleavage Sites of Cas12a Are Modulated by Complementarity between crRNA and DNA

Cas12a is an RNA-guided endonuclease, which displays great potentials and several advantages over the well-known Cas9 in genome editing and engineering. Here, we established a quantitative kinetic scheme to describe the conformational dynamics of Cas12a/crRNA/dsDNA ternary complexes. The highly dyna...

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Detalles Bibliográficos
Autores principales: Zhang, Lujia, Sun, Ruirui, Yang, Mengyi, Peng, Sijia, Cheng, Yongxin, Chen, Chunlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710298/
https://www.ncbi.nlm.nih.gov/pubmed/31437752
http://dx.doi.org/10.1016/j.isci.2019.08.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710298/
https://www.ncbi.nlm.nih.gov/pubmed/31437752
http://dx.doi.org/10.1016/j.isci.2019.08.005

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