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Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during oocyte activation and first cellular division

Nuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into a recipient oocyte to sire a diploid offspring which bears the genome of interest. It requires that the oocyte (maternal) DNA is removed. In fish, because enucleation is difficult to achieve, non-enuc...

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Detalles Bibliográficos
Autores principales: Rouillon, Charlène, Depincé, Alexandra, Chênais, Nathalie, Le Bail, Pierre-Yves, Labbé, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6713701/
https://www.ncbi.nlm.nih.gov/pubmed/31462687
http://dx.doi.org/10.1038/s41598-019-48096-2