Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells

Plant tissue culture is used to grow plant cells, tissues, or organs under sterile and determined conditions on culture media. It is alternative to traditional vegetative propagation, and is applied as an effective technology for the production of valuable secondary metabolites. The Artemisia aucher...

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Autores principales: Abbaspour, Jalil, Ehsanpour, Ali Akbar, Aghaei, Mahmoud, Ghanadian, Mustafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714112/
https://www.ncbi.nlm.nih.gov/pubmed/31516509
http://dx.doi.org/10.4103/1735-5362.263557
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author Abbaspour, Jalil
Ehsanpour, Ali Akbar
Aghaei, Mahmoud
Ghanadian, Mustafa
author_facet Abbaspour, Jalil
Ehsanpour, Ali Akbar
Aghaei, Mahmoud
Ghanadian, Mustafa
author_sort Abbaspour, Jalil
collection PubMed
description Plant tissue culture is used to grow plant cells, tissues, or organs under sterile and determined conditions on culture media. It is alternative to traditional vegetative propagation, and is applied as an effective technology for the production of valuable secondary metabolites. The Artemisia aucheri (A. aucheri) was obtained from shoot culture grown on MS (Murashige and Skoog 1962) medium. Shade-dried aerial parts of in vitro grown A. aucheri (50 g) were extracted with dichloromethane-acetone (90:10). The extract was submitted for isolation to sephadex gel chromatography and preparative thin layer chromatography, which resulted in identification of one known eudesmanolide named artemin or 2,5-dihydroxy-12, 6-eudesmanolide-4(15)-en for the first time in this plant. In cell cytotoxicity test, artemin showed cytotoxic activity against DU-145,LNCaP prostate cancer, and MCF-7 breast cancer cells with IC(50) values of 82.2 ± 5.6, 89.1 ± 6.3 and 111.5 ± 6.7 μM , respectively. Artemin was more active against prostate cancer cells with approximately same cytotoxicity against LNCaP androstane dependent cells and DU 145 which is androstane independent.
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spelling pubmed-67141122019-09-12 Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells Abbaspour, Jalil Ehsanpour, Ali Akbar Aghaei, Mahmoud Ghanadian, Mustafa Res Pharm Sci Original Article Plant tissue culture is used to grow plant cells, tissues, or organs under sterile and determined conditions on culture media. It is alternative to traditional vegetative propagation, and is applied as an effective technology for the production of valuable secondary metabolites. The Artemisia aucheri (A. aucheri) was obtained from shoot culture grown on MS (Murashige and Skoog 1962) medium. Shade-dried aerial parts of in vitro grown A. aucheri (50 g) were extracted with dichloromethane-acetone (90:10). The extract was submitted for isolation to sephadex gel chromatography and preparative thin layer chromatography, which resulted in identification of one known eudesmanolide named artemin or 2,5-dihydroxy-12, 6-eudesmanolide-4(15)-en for the first time in this plant. In cell cytotoxicity test, artemin showed cytotoxic activity against DU-145,LNCaP prostate cancer, and MCF-7 breast cancer cells with IC(50) values of 82.2 ± 5.6, 89.1 ± 6.3 and 111.5 ± 6.7 μM , respectively. Artemin was more active against prostate cancer cells with approximately same cytotoxicity against LNCaP androstane dependent cells and DU 145 which is androstane independent. Wolters Kluwer - Medknow 2019-08 /pmc/articles/PMC6714112/ /pubmed/31516509 http://dx.doi.org/10.4103/1735-5362.263557 Text en Copyright: © 2019 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Abbaspour, Jalil
Ehsanpour, Ali Akbar
Aghaei, Mahmoud
Ghanadian, Mustafa
Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title_full Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title_fullStr Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title_full_unstemmed Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title_short Sesquiterpene lactones from shoot culture of Artemisia aucheri with cytotoxicity against prostate and breast cancer cells
title_sort sesquiterpene lactones from shoot culture of artemisia aucheri with cytotoxicity against prostate and breast cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714112/
https://www.ncbi.nlm.nih.gov/pubmed/31516509
http://dx.doi.org/10.4103/1735-5362.263557
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