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Distinct methylation levels of mature microRNAs in gastrointestinal cancers

The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate their targets in an unvarying fashion. Here we show that a fraction of mature miRNAs including miR-17-5p, -21-5p, and -20...

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Autores principales: Konno, Masamitsu, Koseki, Jun, Asai, Ayumu, Yamagata, Akira, Shimamura, Teppei, Motooka, Daisuke, Okuzaki, Daisuke, Kawamoto, Koichi, Mizushima, Tsunekazu, Eguchi, Hidetoshi, Takiguchi, Shuji, Satoh, Taroh, Mimori, Koshi, Ochiya, Takahiro, Doki, Yuichiro, Ofusa, Ken, Mori, Masaki, Ishii, Hideshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715669/
https://www.ncbi.nlm.nih.gov/pubmed/31467274
http://dx.doi.org/10.1038/s41467-019-11826-1
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author Konno, Masamitsu
Koseki, Jun
Asai, Ayumu
Yamagata, Akira
Shimamura, Teppei
Motooka, Daisuke
Okuzaki, Daisuke
Kawamoto, Koichi
Mizushima, Tsunekazu
Eguchi, Hidetoshi
Takiguchi, Shuji
Satoh, Taroh
Mimori, Koshi
Ochiya, Takahiro
Doki, Yuichiro
Ofusa, Ken
Mori, Masaki
Ishii, Hideshi
author_facet Konno, Masamitsu
Koseki, Jun
Asai, Ayumu
Yamagata, Akira
Shimamura, Teppei
Motooka, Daisuke
Okuzaki, Daisuke
Kawamoto, Koichi
Mizushima, Tsunekazu
Eguchi, Hidetoshi
Takiguchi, Shuji
Satoh, Taroh
Mimori, Koshi
Ochiya, Takahiro
Doki, Yuichiro
Ofusa, Ken
Mori, Masaki
Ishii, Hideshi
author_sort Konno, Masamitsu
collection PubMed
description The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate their targets in an unvarying fashion. Here we show that a fraction of mature miRNAs including miR-17-5p, -21-5p, and -200c-3p and let-7a-5p harbor methyl marks that potentially alter their stability and target recognition. Importantly, methylation of these miRNAs was significantly increased in cancer tissues as compared to paired normal tissues. Furthermore, miR-17-5p methylation level in serum samples distinguished early pancreatic cancer patients from healthy controls with extremely high sensitivity and specificity. These findings provide a basis for diagnostic strategies for early-stage cancer and add a dimension to our understanding of miRNA biology.
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spelling pubmed-67156692019-09-03 Distinct methylation levels of mature microRNAs in gastrointestinal cancers Konno, Masamitsu Koseki, Jun Asai, Ayumu Yamagata, Akira Shimamura, Teppei Motooka, Daisuke Okuzaki, Daisuke Kawamoto, Koichi Mizushima, Tsunekazu Eguchi, Hidetoshi Takiguchi, Shuji Satoh, Taroh Mimori, Koshi Ochiya, Takahiro Doki, Yuichiro Ofusa, Ken Mori, Masaki Ishii, Hideshi Nat Commun Article The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate their targets in an unvarying fashion. Here we show that a fraction of mature miRNAs including miR-17-5p, -21-5p, and -200c-3p and let-7a-5p harbor methyl marks that potentially alter their stability and target recognition. Importantly, methylation of these miRNAs was significantly increased in cancer tissues as compared to paired normal tissues. Furthermore, miR-17-5p methylation level in serum samples distinguished early pancreatic cancer patients from healthy controls with extremely high sensitivity and specificity. These findings provide a basis for diagnostic strategies for early-stage cancer and add a dimension to our understanding of miRNA biology. Nature Publishing Group UK 2019-08-29 /pmc/articles/PMC6715669/ /pubmed/31467274 http://dx.doi.org/10.1038/s41467-019-11826-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Konno, Masamitsu
Koseki, Jun
Asai, Ayumu
Yamagata, Akira
Shimamura, Teppei
Motooka, Daisuke
Okuzaki, Daisuke
Kawamoto, Koichi
Mizushima, Tsunekazu
Eguchi, Hidetoshi
Takiguchi, Shuji
Satoh, Taroh
Mimori, Koshi
Ochiya, Takahiro
Doki, Yuichiro
Ofusa, Ken
Mori, Masaki
Ishii, Hideshi
Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title_full Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title_fullStr Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title_full_unstemmed Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title_short Distinct methylation levels of mature microRNAs in gastrointestinal cancers
title_sort distinct methylation levels of mature micrornas in gastrointestinal cancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6715669/
https://www.ncbi.nlm.nih.gov/pubmed/31467274
http://dx.doi.org/10.1038/s41467-019-11826-1
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