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An automated high throughput solution for DNA extraction and bisulfite-conversion from high volume liquid biopsy specimens: sample preparation for epigenetic analysis
OBJECTIVE: DNA methylation analysis via real-time PCR or other analytical techniques requires purified bisulfite converted DNA. We report on an automated high throughput solution for DNA extraction, bisulfite-conversion, and purification of 96 samples with an input volume of up to 3.5 mL of plasma o...
Autores principales: | Rausch, Sebastian, Hasinger, Oliver, König, Thomas, Schlegel, Anne, Weiss, Gunter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716871/ https://www.ncbi.nlm.nih.gov/pubmed/31470896 http://dx.doi.org/10.1186/s13104-019-4595-3 |
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