PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries

QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method consists of two steps: two parallel asymmetric PCRs, followed by a...

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Detalles Bibliográficos
Autores principales: Jajesniak, Pawel, Tee, Kang Lan, Wong, Tuck Seng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720219/
https://www.ncbi.nlm.nih.gov/pubmed/31405219
http://dx.doi.org/10.3390/ijms20163908

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720219/
https://www.ncbi.nlm.nih.gov/pubmed/31405219
http://dx.doi.org/10.3390/ijms20163908

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