Cargando…

Activation mechanism of the insulin receptor revealed by cryo-EM structure of the fully liganded receptor–ligand complex

Insulin signaling controls metabolic homeostasis. Here, we report the cryo-EM structure of full-length insulin receptor (IR) and insulin complex in the active state. This structure unexpectedly reveals that maximally four insulins can bind the ‘T’-shaped IR dimer at four distinct sites related by 2-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Uchikawa, Emiko, Choi, Eunhee, Shang, Guijun, Yu, Hongtao, Bai, Xiao-chen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2019
Materias:	Structural Biology and Molecular Biophysics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721835/ https://www.ncbi.nlm.nih.gov/pubmed/31436533 http://dx.doi.org/10.7554/eLife.48630

Ejemplares similares

Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands
por: Li, Jie, et al.
Publicado: (2019)

Cryo-EM structure of the human somatostatin receptor 2 complex with its agonist somatostatin delineates the ligand-binding specificity
por: Heo, Yunseok, et al.
Publicado: (2022)

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
por: Zhang, Zhening, et al.
Publicado: (2018)

Cryo-EM structure of respiratory complex I at work
por: Parey, Kristian, et al.
Publicado: (2018)

Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures
por: Rheinberger, Jan, et al.
Publicado: (2018)

Cryo-EM structure of the bifunctional secretin complex of Thermus thermophilus
por: D'Imprima, Edoardo, et al.
Publicado: (2017)

Cryo-EM structure of VASH1-SVBP bound to microtubules
por: Li, Faxiang, et al.
Publicado: (2020)

Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster
por: Agip, Ahmed-Noor A, et al.
Publicado: (2023)

Cryo-EM structure of the endothelin-1-ET(B)-G(i) complex
por: Sano, Fumiya K, et al.
Publicado: (2023)

Ca(2+) inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM
por: Nayak, Ashok R, et al.
Publicado: (2022)

Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1
por: Schrecker, Marina, et al.
Publicado: (2020)

Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2
por: Xie, Yihu, et al.
Publicado: (2021)

Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations
por: Xue, Jing, et al.
Publicado: (2020)

Allosteric inhibition of the T cell receptor by a designed membrane ligand
por: Ye, Yujie, et al.
Publicado: (2023)

Model-based local density sharpening of cryo-EM maps
por: Jakobi, Arjen J, et al.
Publicado: (2017)

Automated systematic evaluation of cryo-EM specimens with SmartScope
por: Bouvette, Jonathan, et al.
Publicado: (2022)

CryoEM structures of open dimers of gyrase A in complex with DNA illuminate mechanism of strand passage
por: Soczek, Katarzyna M, et al.
Publicado: (2018)

Cryo-EM enters a new era
por: Kühlbrandt, Werner
Publicado: (2014)

Cryo-EM structure of the polycystin 2-l1 ion channel
por: Hulse, Raymond E, et al.
Publicado: (2018)

Routine single particle CryoEM sample and grid characterization by tomography
por: Noble, Alex J, et al.
Publicado: (2018)

Cryo-EM analysis of PIP(2) regulation in mammalian GIRK channels
por: Niu, Yiming, et al.
Publicado: (2020)

Cryo-EM structures and functional properties of CALHM channels of the human placenta
por: Drożdżyk, Katarzyna, et al.
Publicado: (2020)

CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin
por: van Pee, Katharina, et al.
Publicado: (2017)

Automated cryo-EM structure refinement using correlation-driven molecular dynamics
por: Igaev, Maxim, et al.
Publicado: (2019)

Mechanism of pharmacochaperoning in a mammalian K(ATP) channel revealed by cryo-EM
por: Martin, Gregory M, et al.
Publicado: (2019)

Cryo-EM structure of the mechanically activated ion channel OSCA1.2
por: Jojoa-Cruz, Sebastian, et al.
Publicado: (2018)

The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1
por: Sosnowski, Piotr, et al.
Publicado: (2018)

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
por: Wang, Ling, et al.
Publicado: (2020)

Cryo-EM structure of the potassium-chloride cotransporter KCC4 in lipid nanodiscs
por: Reid, Michelle S, et al.
Publicado: (2020)

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes
por: Huber, Stefan T, et al.
Publicado: (2022)

Fully automated, sequential focused ion beam milling for cryo-electron tomography
por: Zachs, Tobias, et al.
Publicado: (2020)

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles
por: Bai, Xiao-chen, et al.
Publicado: (2013)

Structural mechanism of ligand activation in human calcium-sensing receptor
por: Geng, Yong, et al.
Publicado: (2016)

Cryo-EM single particle analysis with the Volta phase plate
por: Danev, Radostin, et al.
Publicado: (2016)

New tools for automated high-resolution cryo-EM structure determination in RELION-3
por: Zivanov, Jasenko, et al.
Publicado: (2018)

Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F
por: Alvadia, Carolina, et al.
Publicado: (2019)

Crystal structure of dopamine receptor D4 bound to the subtype selective ligand, L745870
por: Zhou, Ye, et al.
Publicado: (2019)

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION
por: Nakane, Takanori, et al.
Publicado: (2018)

Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule
por: Lacey, Samuel E, et al.
Publicado: (2019)

The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanism
por: Stanisich, Jessica J, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_8gitnivsnll3rc712fvs5hei6u