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Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications

Epidemiological studies identified raw cow’s milk consumption as an important environmental exposure that prevents allergic diseases. In the present study, we investigated whether raw cow’s milk has the capacity to induce tolerance to an unrelated, non-milk, food allergen. Histone acetylation of T c...

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Autores principales: Abbring, Suzanne, Wolf, Johanna, Ayechu-Muruzabal, Veronica, Diks, Mara A.P., Alashkar Alhamwe, Bilal, Alhamdan, Fahd, Harb, Hani, Renz, Harald, Garn, Holger, Garssen, Johan, Potaczek, Daniel P., van Esch, Betty C.A.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723026/
https://www.ncbi.nlm.nih.gov/pubmed/31349704
http://dx.doi.org/10.3390/nu11081721
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author Abbring, Suzanne
Wolf, Johanna
Ayechu-Muruzabal, Veronica
Diks, Mara A.P.
Alashkar Alhamwe, Bilal
Alhamdan, Fahd
Harb, Hani
Renz, Harald
Garn, Holger
Garssen, Johan
Potaczek, Daniel P.
van Esch, Betty C.A.M.
author_facet Abbring, Suzanne
Wolf, Johanna
Ayechu-Muruzabal, Veronica
Diks, Mara A.P.
Alashkar Alhamwe, Bilal
Alhamdan, Fahd
Harb, Hani
Renz, Harald
Garn, Holger
Garssen, Johan
Potaczek, Daniel P.
van Esch, Betty C.A.M.
author_sort Abbring, Suzanne
collection PubMed
description Epidemiological studies identified raw cow’s milk consumption as an important environmental exposure that prevents allergic diseases. In the present study, we investigated whether raw cow’s milk has the capacity to induce tolerance to an unrelated, non-milk, food allergen. Histone acetylation of T cell genes was investigated to assess potential epigenetic regulation. Female C3H/HeOuJ mice were sensitized and challenged to ovalbumin. Prior to sensitization, the mice were treated with raw milk, processed milk, or phosphate-buffered saline for eight days. Allergic symptoms were assessed after challenge and histone modifications in T cell-related genes of splenocyte-derived CD4(+) T cells and the mesenteric lymph nodes were analyzed after milk exposure and after challenge. Unlike processed milk, raw milk decreased allergic symptoms. After raw milk exposure, histone acetylation of Th1-, Th2-, and regulatory T cell-related genes of splenocyte-derived CD4(+) T cells was higher than after processed milk exposure. After allergy induction, this general immune stimulation was resolved and histone acetylation of Th2 genes was lower when compared to processed milk. Raw milk reduces allergic symptoms to an unrelated, non-milk, food allergen in a murine model for food allergy. The activation of T cell-related genes could be responsible for the observed tolerance induction, which suggested that epigenetic modifications contribute to the allergy-protective effect of raw milk.
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spelling pubmed-67230262019-09-10 Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications Abbring, Suzanne Wolf, Johanna Ayechu-Muruzabal, Veronica Diks, Mara A.P. Alashkar Alhamwe, Bilal Alhamdan, Fahd Harb, Hani Renz, Harald Garn, Holger Garssen, Johan Potaczek, Daniel P. van Esch, Betty C.A.M. Nutrients Article Epidemiological studies identified raw cow’s milk consumption as an important environmental exposure that prevents allergic diseases. In the present study, we investigated whether raw cow’s milk has the capacity to induce tolerance to an unrelated, non-milk, food allergen. Histone acetylation of T cell genes was investigated to assess potential epigenetic regulation. Female C3H/HeOuJ mice were sensitized and challenged to ovalbumin. Prior to sensitization, the mice were treated with raw milk, processed milk, or phosphate-buffered saline for eight days. Allergic symptoms were assessed after challenge and histone modifications in T cell-related genes of splenocyte-derived CD4(+) T cells and the mesenteric lymph nodes were analyzed after milk exposure and after challenge. Unlike processed milk, raw milk decreased allergic symptoms. After raw milk exposure, histone acetylation of Th1-, Th2-, and regulatory T cell-related genes of splenocyte-derived CD4(+) T cells was higher than after processed milk exposure. After allergy induction, this general immune stimulation was resolved and histone acetylation of Th2 genes was lower when compared to processed milk. Raw milk reduces allergic symptoms to an unrelated, non-milk, food allergen in a murine model for food allergy. The activation of T cell-related genes could be responsible for the observed tolerance induction, which suggested that epigenetic modifications contribute to the allergy-protective effect of raw milk. MDPI 2019-07-25 /pmc/articles/PMC6723026/ /pubmed/31349704 http://dx.doi.org/10.3390/nu11081721 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abbring, Suzanne
Wolf, Johanna
Ayechu-Muruzabal, Veronica
Diks, Mara A.P.
Alashkar Alhamwe, Bilal
Alhamdan, Fahd
Harb, Hani
Renz, Harald
Garn, Holger
Garssen, Johan
Potaczek, Daniel P.
van Esch, Betty C.A.M.
Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title_full Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title_fullStr Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title_full_unstemmed Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title_short Raw Cow’s Milk Reduces Allergic Symptoms in a Murine Model for Food Allergy—A Potential Role for Epigenetic Modifications
title_sort raw cow’s milk reduces allergic symptoms in a murine model for food allergy—a potential role for epigenetic modifications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723026/
https://www.ncbi.nlm.nih.gov/pubmed/31349704
http://dx.doi.org/10.3390/nu11081721
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