Cargando…
Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice
Oleacein is one of the most abundant polyphenolic compounds of olive oil, which has been shown to play a protective role against several metabolic abnormalities, including dyslipidemia, insulin resistance, and glucose intolerance. Herein, we investigated the effects of oleacein on certain markers of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723526/ https://www.ncbi.nlm.nih.gov/pubmed/31394876 http://dx.doi.org/10.3390/nu11081829 |
_version_ | 1783448789404614656 |
---|---|
author | Lepore, Saverio Massimo Maggisano, Valentina Bulotta, Stefania Mignogna, Chiara Arcidiacono, Biagio Procopio, Antonio Brunetti, Antonio Russo, Diego Celano, Marilena |
author_facet | Lepore, Saverio Massimo Maggisano, Valentina Bulotta, Stefania Mignogna, Chiara Arcidiacono, Biagio Procopio, Antonio Brunetti, Antonio Russo, Diego Celano, Marilena |
author_sort | Lepore, Saverio Massimo |
collection | PubMed |
description | Oleacein is one of the most abundant polyphenolic compounds of olive oil, which has been shown to play a protective role against several metabolic abnormalities, including dyslipidemia, insulin resistance, and glucose intolerance. Herein, we investigated the effects of oleacein on certain markers of adipogenesis and insulin-resistance in vitro, in 3T3-L1 adipocytes, and in vivo in high-fat diet (HFD)-fed mice. During the differentiation process of 3T3-L1 preadipocytes into adipocytes, oleacein strongly inhibited lipid accumulation, and decreased protein levels of peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid synthase (FAS), while increasing Adiponectin levels. In vivo, treatment with oleacein of C57BL/6JOlaHsd mice fed with HFD for 5 and 13 weeks prevented the increase in adipocyte size and reduced the inflammatory infiltration of macrophages and lymphocytes in adipose tissue. These effects were accompanied by changes in the expression of adipose tissue-specific regulatory elements such as PPARγ, FAS, sterol regulatory element-binding transcription factor-1 (SREBP-1), and Adiponectin, while the expression of insulin-sensitive muscle/fat glucose transporter Glut-4 was restored in HFD-fed mice treated with oleacein. Collectively, our findings indicate that protection against HFD-induced adiposity by oleacein in mice is mediated by the modulation of regulators of adipogenesis. Protection against HFD-induced obesity is effective in improving peripheral insulin sensitivity. |
format | Online Article Text |
id | pubmed-6723526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67235262019-09-10 Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice Lepore, Saverio Massimo Maggisano, Valentina Bulotta, Stefania Mignogna, Chiara Arcidiacono, Biagio Procopio, Antonio Brunetti, Antonio Russo, Diego Celano, Marilena Nutrients Article Oleacein is one of the most abundant polyphenolic compounds of olive oil, which has been shown to play a protective role against several metabolic abnormalities, including dyslipidemia, insulin resistance, and glucose intolerance. Herein, we investigated the effects of oleacein on certain markers of adipogenesis and insulin-resistance in vitro, in 3T3-L1 adipocytes, and in vivo in high-fat diet (HFD)-fed mice. During the differentiation process of 3T3-L1 preadipocytes into adipocytes, oleacein strongly inhibited lipid accumulation, and decreased protein levels of peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid synthase (FAS), while increasing Adiponectin levels. In vivo, treatment with oleacein of C57BL/6JOlaHsd mice fed with HFD for 5 and 13 weeks prevented the increase in adipocyte size and reduced the inflammatory infiltration of macrophages and lymphocytes in adipose tissue. These effects were accompanied by changes in the expression of adipose tissue-specific regulatory elements such as PPARγ, FAS, sterol regulatory element-binding transcription factor-1 (SREBP-1), and Adiponectin, while the expression of insulin-sensitive muscle/fat glucose transporter Glut-4 was restored in HFD-fed mice treated with oleacein. Collectively, our findings indicate that protection against HFD-induced adiposity by oleacein in mice is mediated by the modulation of regulators of adipogenesis. Protection against HFD-induced obesity is effective in improving peripheral insulin sensitivity. MDPI 2019-08-07 /pmc/articles/PMC6723526/ /pubmed/31394876 http://dx.doi.org/10.3390/nu11081829 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lepore, Saverio Massimo Maggisano, Valentina Bulotta, Stefania Mignogna, Chiara Arcidiacono, Biagio Procopio, Antonio Brunetti, Antonio Russo, Diego Celano, Marilena Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title | Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title_full | Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title_fullStr | Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title_full_unstemmed | Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title_short | Oleacein Prevents High Fat Diet-Induced Adiposity and Ameliorates Some Biochemical Parameters of Insulin Sensitivity in Mice |
title_sort | oleacein prevents high fat diet-induced adiposity and ameliorates some biochemical parameters of insulin sensitivity in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723526/ https://www.ncbi.nlm.nih.gov/pubmed/31394876 http://dx.doi.org/10.3390/nu11081829 |
work_keys_str_mv | AT leporesaveriomassimo oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT maggisanovalentina oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT bulottastefania oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT mignognachiara oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT arcidiaconobiagio oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT procopioantonio oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT brunettiantonio oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT russodiego oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice AT celanomarilena oleaceinpreventshighfatdietinducedadiposityandamelioratessomebiochemicalparametersofinsulinsensitivityinmice |