Cargando…

Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate

Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from natural products have shown a blood pressure lowering effect with no side effects. In this study, two novel ACE inhibitory peptides (His-Leu-His-Thr, HLHT and Gly-Trp-Ala, GWA) were purified from pearl oyster (Pinctada fucata mar...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Pengru, Lan, Xiongdiao, Yaseen, Muhammad, Wu, Shanguang, Feng, Xuezhen, Zhou, Liqin, Sun, Jianhua, Liao, Anping, Liao, Dankui, Sun, Lixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723713/
https://www.ncbi.nlm.nih.gov/pubmed/31398788
http://dx.doi.org/10.3390/md17080463
_version_ 1783448834145255424
author Liu, Pengru
Lan, Xiongdiao
Yaseen, Muhammad
Wu, Shanguang
Feng, Xuezhen
Zhou, Liqin
Sun, Jianhua
Liao, Anping
Liao, Dankui
Sun, Lixia
author_facet Liu, Pengru
Lan, Xiongdiao
Yaseen, Muhammad
Wu, Shanguang
Feng, Xuezhen
Zhou, Liqin
Sun, Jianhua
Liao, Anping
Liao, Dankui
Sun, Lixia
author_sort Liu, Pengru
collection PubMed
description Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from natural products have shown a blood pressure lowering effect with no side effects. In this study, two novel ACE inhibitory peptides (His-Leu-His-Thr, HLHT and Gly-Trp-Ala, GWA) were purified from pearl oyster (Pinctada fucata martensii) meat protein hydrolysate with alkaline protease by ultrafiltration, polyethylene glycol methyl ether modified immobilized metal ion affinity medium, and reverse-phase high performance liquid chromatography. Both peptides exhibited high ACE inhibitory activity with IC(50) values of 458.06 ± 3.24 μM and 109.25 ± 1.45 μM, respectively. Based on the results of a Lineweaver-Burk plot, HLHT and GWA were found to be non-competitive inhibitor and competitive inhibitor respectively, which were confirmed by molecular docking. Furthermore, the pearl oyster meat protein hydrolysate exhibited an effective antihypertensive effect on SD rats. These results conclude that pearl oyster meat protein is a potential resource of ACE inhibitory peptides and the purified peptides, HLHT and GWA, can be exploited as functional food ingredients against hypertension.
format Online
Article
Text
id pubmed-6723713
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67237132019-09-10 Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate Liu, Pengru Lan, Xiongdiao Yaseen, Muhammad Wu, Shanguang Feng, Xuezhen Zhou, Liqin Sun, Jianhua Liao, Anping Liao, Dankui Sun, Lixia Mar Drugs Article Angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from natural products have shown a blood pressure lowering effect with no side effects. In this study, two novel ACE inhibitory peptides (His-Leu-His-Thr, HLHT and Gly-Trp-Ala, GWA) were purified from pearl oyster (Pinctada fucata martensii) meat protein hydrolysate with alkaline protease by ultrafiltration, polyethylene glycol methyl ether modified immobilized metal ion affinity medium, and reverse-phase high performance liquid chromatography. Both peptides exhibited high ACE inhibitory activity with IC(50) values of 458.06 ± 3.24 μM and 109.25 ± 1.45 μM, respectively. Based on the results of a Lineweaver-Burk plot, HLHT and GWA were found to be non-competitive inhibitor and competitive inhibitor respectively, which were confirmed by molecular docking. Furthermore, the pearl oyster meat protein hydrolysate exhibited an effective antihypertensive effect on SD rats. These results conclude that pearl oyster meat protein is a potential resource of ACE inhibitory peptides and the purified peptides, HLHT and GWA, can be exploited as functional food ingredients against hypertension. MDPI 2019-08-08 /pmc/articles/PMC6723713/ /pubmed/31398788 http://dx.doi.org/10.3390/md17080463 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Pengru
Lan, Xiongdiao
Yaseen, Muhammad
Wu, Shanguang
Feng, Xuezhen
Zhou, Liqin
Sun, Jianhua
Liao, Anping
Liao, Dankui
Sun, Lixia
Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title_full Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title_fullStr Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title_full_unstemmed Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title_short Purification, Characterization and Evaluation of Inhibitory Mechanism of ACE Inhibitory Peptides from Pearl Oyster (Pinctada fucata martensii) Meat Protein Hydrolysate
title_sort purification, characterization and evaluation of inhibitory mechanism of ace inhibitory peptides from pearl oyster (pinctada fucata martensii) meat protein hydrolysate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723713/
https://www.ncbi.nlm.nih.gov/pubmed/31398788
http://dx.doi.org/10.3390/md17080463
work_keys_str_mv AT liupengru purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT lanxiongdiao purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT yaseenmuhammad purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT wushanguang purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT fengxuezhen purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT zhouliqin purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT sunjianhua purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT liaoanping purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT liaodankui purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate
AT sunlixia purificationcharacterizationandevaluationofinhibitorymechanismofaceinhibitorypeptidesfrompearloysterpinctadafucatamartensiimeatproteinhydrolysate