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Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-pote...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723998/ https://www.ncbi.nlm.nih.gov/pubmed/31349695 http://dx.doi.org/10.3390/md17080438 |
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author | Liu, Xiaoyang Wang, Zixu Yin, Fawen Liu, Yuxin Qin, Ningbo Nakamura, Yoshimasa Shahidi, Fereidoon Yu, Chenxu Zhou, Dayong Zhu, Beiwei |
author_facet | Liu, Xiaoyang Wang, Zixu Yin, Fawen Liu, Yuxin Qin, Ningbo Nakamura, Yoshimasa Shahidi, Fereidoon Yu, Chenxu Zhou, Dayong Zhu, Beiwei |
author_sort | Liu, Xiaoyang |
collection | PubMed |
description | In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides. |
format | Online Article Text |
id | pubmed-6723998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67239982019-09-10 Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides Liu, Xiaoyang Wang, Zixu Yin, Fawen Liu, Yuxin Qin, Ningbo Nakamura, Yoshimasa Shahidi, Fereidoon Yu, Chenxu Zhou, Dayong Zhu, Beiwei Mar Drugs Article In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides. MDPI 2019-07-25 /pmc/articles/PMC6723998/ /pubmed/31349695 http://dx.doi.org/10.3390/md17080438 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xiaoyang Wang, Zixu Yin, Fawen Liu, Yuxin Qin, Ningbo Nakamura, Yoshimasa Shahidi, Fereidoon Yu, Chenxu Zhou, Dayong Zhu, Beiwei Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title | Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title_full | Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title_fullStr | Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title_full_unstemmed | Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title_short | Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides |
title_sort | zinc-chelating mechanism of sea cucumber (stichopus japonicus)-derived synthetic peptides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723998/ https://www.ncbi.nlm.nih.gov/pubmed/31349695 http://dx.doi.org/10.3390/md17080438 |
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