Cargando…

Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides

In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-pote...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiaoyang, Wang, Zixu, Yin, Fawen, Liu, Yuxin, Qin, Ningbo, Nakamura, Yoshimasa, Shahidi, Fereidoon, Yu, Chenxu, Zhou, Dayong, Zhu, Beiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723998/
https://www.ncbi.nlm.nih.gov/pubmed/31349695
http://dx.doi.org/10.3390/md17080438
_version_ 1783448901385191424
author Liu, Xiaoyang
Wang, Zixu
Yin, Fawen
Liu, Yuxin
Qin, Ningbo
Nakamura, Yoshimasa
Shahidi, Fereidoon
Yu, Chenxu
Zhou, Dayong
Zhu, Beiwei
author_facet Liu, Xiaoyang
Wang, Zixu
Yin, Fawen
Liu, Yuxin
Qin, Ningbo
Nakamura, Yoshimasa
Shahidi, Fereidoon
Yu, Chenxu
Zhou, Dayong
Zhu, Beiwei
author_sort Liu, Xiaoyang
collection PubMed
description In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides.
format Online
Article
Text
id pubmed-6723998
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67239982019-09-10 Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides Liu, Xiaoyang Wang, Zixu Yin, Fawen Liu, Yuxin Qin, Ningbo Nakamura, Yoshimasa Shahidi, Fereidoon Yu, Chenxu Zhou, Dayong Zhu, Beiwei Mar Drugs Article In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides. MDPI 2019-07-25 /pmc/articles/PMC6723998/ /pubmed/31349695 http://dx.doi.org/10.3390/md17080438 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiaoyang
Wang, Zixu
Yin, Fawen
Liu, Yuxin
Qin, Ningbo
Nakamura, Yoshimasa
Shahidi, Fereidoon
Yu, Chenxu
Zhou, Dayong
Zhu, Beiwei
Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title_full Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title_fullStr Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title_full_unstemmed Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title_short Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides
title_sort zinc-chelating mechanism of sea cucumber (stichopus japonicus)-derived synthetic peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723998/
https://www.ncbi.nlm.nih.gov/pubmed/31349695
http://dx.doi.org/10.3390/md17080438
work_keys_str_mv AT liuxiaoyang zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT wangzixu zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT yinfawen zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT liuyuxin zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT qinningbo zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT nakamurayoshimasa zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT shahidifereidoon zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT yuchenxu zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT zhoudayong zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides
AT zhubeiwei zincchelatingmechanismofseacucumberstichopusjaponicusderivedsyntheticpeptides