Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM

How membrane proteins oligomerize determines their function. Superresolution microscopy can report on protein clustering and extract quantitative molecular information. Here, we evaluate the blinking kinetics of four photoactivatable fluorescent proteins for quantitative single-molecule microscopy....

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Detalles Bibliográficos
Autores principales: Baldering, Tim N., Dietz, Marina S., Gatterdam, Karl, Karathanasis, Christos, Wieneke, Ralph, Tampé, Robert, Heilemann, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724688/
https://www.ncbi.nlm.nih.gov/pubmed/30969885
http://dx.doi.org/10.1091/mbc.E18-10-0661

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724688/
https://www.ncbi.nlm.nih.gov/pubmed/30969885
http://dx.doi.org/10.1091/mbc.E18-10-0661

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