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Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM
How membrane proteins oligomerize determines their function. Superresolution microscopy can report on protein clustering and extract quantitative molecular information. Here, we evaluate the blinking kinetics of four photoactivatable fluorescent proteins for quantitative single-molecule microscopy....
Autores principales: | Baldering, Tim N., Dietz, Marina S., Gatterdam, Karl, Karathanasis, Christos, Wieneke, Ralph, Tampé, Robert, Heilemann, Mike |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724688/ https://www.ncbi.nlm.nih.gov/pubmed/30969885 http://dx.doi.org/10.1091/mbc.E18-10-0661 |
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