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Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes

Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulate...

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Autores principales: Pipaliya, Shweta V., Schlacht, Alexander, Klinger, Christen M., Kahn, Richard A., Dacks, Joel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727740/
https://www.ncbi.nlm.nih.gov/pubmed/31141460
http://dx.doi.org/10.1091/mbc.E19-01-0073
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author Pipaliya, Shweta V.
Schlacht, Alexander
Klinger, Christen M.
Kahn, Richard A.
Dacks, Joel
author_facet Pipaliya, Shweta V.
Schlacht, Alexander
Klinger, Christen M.
Kahn, Richard A.
Dacks, Joel
author_sort Pipaliya, Shweta V.
collection PubMed
description Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulated by ADP-ribosylation factor (ARF) GTPases and the development throughout eukaryotic evolution of increasingly complex systems of such traffic required the acquisition of a functionally diverse cohort of ARF GEFs to control it. We performed phylogenetic analyses of ARF GEFs in eukaryotes, defined by the presence of the Sec7 domain, and found three subfamilies (BIG, GBF1, and cytohesins) to have been present in the ancestor of all eukaryotes. The four other subfamilies (EFA6/PSD, IQSEC7/BRAG, FBX8, and TBS) are opisthokont, holozoan, metazoan, and alveolate/haptophyte specific, respectively, and each is derived from cytohesins. We also identified a cytohesin-derived subfamily, termed ankyrin repeat-containing cytohesin, that independently evolved in amoebozoans and members of the SAR and haptophyte clades. Building on evolutionary data for the ARF family GTPases and their GTPase-­activating proteins allowed the generation of hypotheses about ARF GEF protein function(s) as well as a better understanding of the origins and evolution of cellular complexity in eukaryotes.
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spelling pubmed-67277402019-09-30 Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes Pipaliya, Shweta V. Schlacht, Alexander Klinger, Christen M. Kahn, Richard A. Dacks, Joel Mol Biol Cell Articles Guanine nucleotide exchange factors (GEFs) are the initiators of signaling by every regulatory GTPase, which in turn act to regulate a wide array of essential cellular processes. To date, each family of GTPases is activated by distinct families of GEFs. Bidirectional membrane trafficking is regulated by ADP-ribosylation factor (ARF) GTPases and the development throughout eukaryotic evolution of increasingly complex systems of such traffic required the acquisition of a functionally diverse cohort of ARF GEFs to control it. We performed phylogenetic analyses of ARF GEFs in eukaryotes, defined by the presence of the Sec7 domain, and found three subfamilies (BIG, GBF1, and cytohesins) to have been present in the ancestor of all eukaryotes. The four other subfamilies (EFA6/PSD, IQSEC7/BRAG, FBX8, and TBS) are opisthokont, holozoan, metazoan, and alveolate/haptophyte specific, respectively, and each is derived from cytohesins. We also identified a cytohesin-derived subfamily, termed ankyrin repeat-containing cytohesin, that independently evolved in amoebozoans and members of the SAR and haptophyte clades. Building on evolutionary data for the ARF family GTPases and their GTPase-­activating proteins allowed the generation of hypotheses about ARF GEF protein function(s) as well as a better understanding of the origins and evolution of cellular complexity in eukaryotes. The American Society for Cell Biology 2019-07-15 /pmc/articles/PMC6727740/ /pubmed/31141460 http://dx.doi.org/10.1091/mbc.E19-01-0073 Text en © 2019 Pipaliya et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Pipaliya, Shweta V.
Schlacht, Alexander
Klinger, Christen M.
Kahn, Richard A.
Dacks, Joel
Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title_full Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title_fullStr Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title_full_unstemmed Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title_short Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes
title_sort ancient complement and lineage-specific evolution of the sec7 arf gef proteins in eukaryotes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727740/
https://www.ncbi.nlm.nih.gov/pubmed/31141460
http://dx.doi.org/10.1091/mbc.E19-01-0073
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