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Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)

Methods to detect protozoa are needed for food safety monitoring. We evaluated protocols to recover Giardia spp. cysts in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) and then detection was performed by concentrating with formalin/ether solutions and microscopy or immunofluorescence or D...

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Autores principales: Hernández-Arango, Natalia, Pinto, Valeria, Muñoz-Sanchez, Deicy, Lora-Suarez, Fabiana, Gómez-Marín, Jorge Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728726/
https://www.ncbi.nlm.nih.gov/pubmed/31517100
http://dx.doi.org/10.1016/j.heliyon.2019.e02377
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author Hernández-Arango, Natalia
Pinto, Valeria
Muñoz-Sanchez, Deicy
Lora-Suarez, Fabiana
Gómez-Marín, Jorge Enrique
author_facet Hernández-Arango, Natalia
Pinto, Valeria
Muñoz-Sanchez, Deicy
Lora-Suarez, Fabiana
Gómez-Marín, Jorge Enrique
author_sort Hernández-Arango, Natalia
collection PubMed
description Methods to detect protozoa are needed for food safety monitoring. We evaluated protocols to recover Giardia spp. cysts in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) and then detection was performed by concentrating with formalin/ether solutions and microscopy or immunofluorescence or DNA amplification via PCR. To evaluate this methodology, G. duodenalis cysts were inoculated in triplicate (10 cysts) in 35-g samples of lettuce and cabbage. The method obtaining the highest percentage of recovery in cabbage was sulfamic acid solution plus stirring with stomacher (47.7% ± 7.5). For lettuce, the best method was glycine solution plus stirring with stomacher (46.6% ± 5.3). Inter-observer agreement was of 0.99. Giardia was detected by amplifying specific sequences for the DNA coding SSU rRNA. In 27 lettuce samples and 27 cabbage samples, obtained from supermarkets and street vendors, two lettuce samples (7.4%) and one cabbage sample (3.7%) were positive for Giardia via PCR assay and were sequenced, determining that they were two of assemblage B and one of lettuce to assemblage E. This method is proposed to detect Giardia in vegetables by PCR detection, enabling public health authorities to identify genotypes circulating in food, which will help to establish measures that reduce outbreaks of parasitic diseases associated with contaminated food.
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spelling pubmed-67287262019-09-12 Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) Hernández-Arango, Natalia Pinto, Valeria Muñoz-Sanchez, Deicy Lora-Suarez, Fabiana Gómez-Marín, Jorge Enrique Heliyon Article Methods to detect protozoa are needed for food safety monitoring. We evaluated protocols to recover Giardia spp. cysts in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) and then detection was performed by concentrating with formalin/ether solutions and microscopy or immunofluorescence or DNA amplification via PCR. To evaluate this methodology, G. duodenalis cysts were inoculated in triplicate (10 cysts) in 35-g samples of lettuce and cabbage. The method obtaining the highest percentage of recovery in cabbage was sulfamic acid solution plus stirring with stomacher (47.7% ± 7.5). For lettuce, the best method was glycine solution plus stirring with stomacher (46.6% ± 5.3). Inter-observer agreement was of 0.99. Giardia was detected by amplifying specific sequences for the DNA coding SSU rRNA. In 27 lettuce samples and 27 cabbage samples, obtained from supermarkets and street vendors, two lettuce samples (7.4%) and one cabbage sample (3.7%) were positive for Giardia via PCR assay and were sequenced, determining that they were two of assemblage B and one of lettuce to assemblage E. This method is proposed to detect Giardia in vegetables by PCR detection, enabling public health authorities to identify genotypes circulating in food, which will help to establish measures that reduce outbreaks of parasitic diseases associated with contaminated food. Elsevier 2019-08-28 /pmc/articles/PMC6728726/ /pubmed/31517100 http://dx.doi.org/10.1016/j.heliyon.2019.e02377 Text en © 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hernández-Arango, Natalia
Pinto, Valeria
Muñoz-Sanchez, Deicy
Lora-Suarez, Fabiana
Gómez-Marín, Jorge Enrique
Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title_full Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title_fullStr Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title_full_unstemmed Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title_short Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce)
title_sort detection of giardia spp. with formalin/ether concentration in brassica oleracea (cabbage) and lactuca sativa (lettuce)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728726/
https://www.ncbi.nlm.nih.gov/pubmed/31517100
http://dx.doi.org/10.1016/j.heliyon.2019.e02377
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