Cargando…

A novel method to rescue and culture duck Astrovirus type 1 in vitro

BACKGROUND: Reverse genetics systems enable the manipulation of viral genomes and therefore serve as robust reverse genetic tools to study RNA viruses. A DNA-launched rescue system initiates the transcription of viral genomic cDNA from eukaryotic promoter in transfected cells, generating homogenous...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ruihua, Lan, Jingjing, Li, Haie, Chen, Junhao, Yang, Yupeng, Lin, Shaoli, Xie, Zhijing, Jiang, Shijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6729042/
https://www.ncbi.nlm.nih.gov/pubmed/31488178
http://dx.doi.org/10.1186/s12985-019-1218-5