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Development of a new extraction method based on high-intensity ultra-sonication to study RNA regulation of the filamentous cyanobacteria Planktothrix

Efficient RNA extraction methods are needed to study transcript regulation. Such methods must lyse the cell without degrading the genetic material. For cyanobacteria this can be particularly challenging because of the presence of the cyanobacteria cell envelope. The great breath of cyanobacterial sh...

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Detalles Bibliográficos
Autores principales: Kim Tiam, Sandra, Comte, Katia, Dalle, Caroline, Duval, Charlotte, Pancrace, Claire, Gugger, Muriel, Marie, Benjamin, Yéprémian, Claude, Bernard, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730872/
https://www.ncbi.nlm.nih.gov/pubmed/31490972
http://dx.doi.org/10.1371/journal.pone.0222029