Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery

An early bottleneck in the rapid isolation of new antibody fragment binders using in vitro library approaches is the inertia encountered in acquiring and preparing soluble antigen fragments. In this report, we describe a simple, yet powerful strategy that exploits the properties of the SpyCatcher/Sp...

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Detalles Bibliográficos
Autores principales: Fierle, Julie K., Abram-Saliba, Johan, Brioschi, Matteo, deTiani, Mariastella, Coukos, George, Dunn, Steven M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731262/
https://www.ncbi.nlm.nih.gov/pubmed/31492910
http://dx.doi.org/10.1038/s41598-019-49233-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731262/
https://www.ncbi.nlm.nih.gov/pubmed/31492910
http://dx.doi.org/10.1038/s41598-019-49233-7

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