Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated. Here, we investigate Cas9, dCas9, and CRISPRi/a off-target activity in screens for essent...

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Detalles Bibliográficos
Autores principales: Tycko, Josh, Wainberg, Michael, Marinov, Georgi K., Ursu, Oana, Hess, Gaelen T., Ego, Braeden K., Aradhana, Li, Amy, Truong, Alisa, Trevino, Alexandro E., Spees, Kaitlyn, Yao, David, Kaplow, Irene M., Greenside, Peyton G., Morgens, David W., Phanstiel, Douglas H., Snyder, Michael P., Bintu, Lacramioara, Greenleaf, William J., Kundaje, Anshul, Bassik, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731277/
https://www.ncbi.nlm.nih.gov/pubmed/31492858
http://dx.doi.org/10.1038/s41467-019-11955-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731277/
https://www.ncbi.nlm.nih.gov/pubmed/31492858
http://dx.doi.org/10.1038/s41467-019-11955-7

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