High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China

Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationshi...

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Autores principales: Manawasinghe, Ishara S., Dissanayake, Asha J., Li, Xinghong, Liu, Mei, Wanasinghe, Dhanushka N., Xu, Jianping, Zhao, Wensheng, Zhang, Wei, Zhou, Yueyan, Hyde, Kevin D., Brooks, Siraprapa, Yan, Jiye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732904/
https://www.ncbi.nlm.nih.gov/pubmed/31543868
http://dx.doi.org/10.3389/fmicb.2019.01936
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author Manawasinghe, Ishara S.
Dissanayake, Asha J.
Li, Xinghong
Liu, Mei
Wanasinghe, Dhanushka N.
Xu, Jianping
Zhao, Wensheng
Zhang, Wei
Zhou, Yueyan
Hyde, Kevin D.
Brooks, Siraprapa
Yan, Jiye
author_facet Manawasinghe, Ishara S.
Dissanayake, Asha J.
Li, Xinghong
Liu, Mei
Wanasinghe, Dhanushka N.
Xu, Jianping
Zhao, Wensheng
Zhang, Wei
Zhou, Yueyan
Hyde, Kevin D.
Brooks, Siraprapa
Yan, Jiye
author_sort Manawasinghe, Ishara S.
collection PubMed
description Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize Diaporthe species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, Diaporthe guangxiensis (D. guangxiensis), Diaporthe hubeiensis (D. hubeiensis), Diaporthe viniferae (D. viniferae), and three new host records, Diaporthe gulyae (D. gulyae), Diaporthe pescicola (D. pescicola), and Diaporthe unshiuensis (D. unshiuensis). The most commonly isolated species was Diaporthe eres (D. eres). In addition, high genetic diversity was observed for D. eres in Chinese vineyards. Haplotype network analysis of D. eres isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, D. gulyae was the most aggressive taxon, whereas D. hubeiensis was the least aggressive. This study provides new insights into the Diaporthe species associated with grapevines in China, and our results can be used to develop effective disease management strategies.
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spelling pubmed-67329042019-09-20 High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China Manawasinghe, Ishara S. Dissanayake, Asha J. Li, Xinghong Liu, Mei Wanasinghe, Dhanushka N. Xu, Jianping Zhao, Wensheng Zhang, Wei Zhou, Yueyan Hyde, Kevin D. Brooks, Siraprapa Yan, Jiye Front Microbiol Microbiology Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize Diaporthe species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, Diaporthe guangxiensis (D. guangxiensis), Diaporthe hubeiensis (D. hubeiensis), Diaporthe viniferae (D. viniferae), and three new host records, Diaporthe gulyae (D. gulyae), Diaporthe pescicola (D. pescicola), and Diaporthe unshiuensis (D. unshiuensis). The most commonly isolated species was Diaporthe eres (D. eres). In addition, high genetic diversity was observed for D. eres in Chinese vineyards. Haplotype network analysis of D. eres isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, D. gulyae was the most aggressive taxon, whereas D. hubeiensis was the least aggressive. This study provides new insights into the Diaporthe species associated with grapevines in China, and our results can be used to develop effective disease management strategies. Frontiers Media S.A. 2019-09-02 /pmc/articles/PMC6732904/ /pubmed/31543868 http://dx.doi.org/10.3389/fmicb.2019.01936 Text en Copyright © 2019 Manawasinghe, Dissanayake, Li, Liu, Wanasinghe, Xu, Zhao, Zhang, Zhou, Hyde, Brooks and Yan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Manawasinghe, Ishara S.
Dissanayake, Asha J.
Li, Xinghong
Liu, Mei
Wanasinghe, Dhanushka N.
Xu, Jianping
Zhao, Wensheng
Zhang, Wei
Zhou, Yueyan
Hyde, Kevin D.
Brooks, Siraprapa
Yan, Jiye
High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title_full High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title_fullStr High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title_full_unstemmed High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title_short High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China
title_sort high genetic diversity and species complexity of diaporthe associated with grapevine dieback in china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732904/
https://www.ncbi.nlm.nih.gov/pubmed/31543868
http://dx.doi.org/10.3389/fmicb.2019.01936
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