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Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions

Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locatio...

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Detalles Bibliográficos
Autores principales: Jaiswal, Astha, Hoerth, Christian H., Zúñiga Pereira, Ana M., Lorenz, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733880/
https://www.ncbi.nlm.nih.gov/pubmed/31501484
http://dx.doi.org/10.1038/s41598-019-49408-2