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Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions
Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locatio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733880/ https://www.ncbi.nlm.nih.gov/pubmed/31501484 http://dx.doi.org/10.1038/s41598-019-49408-2 |