Crisflash: open-source software to generate CRISPR guide RNAs against genomes annotated with individual variation

SUMMARY: CRISPR/Cas9 system requires short guide RNAs (sgRNAs) to direct genome modification. Most currently available tools for sgRNA design operate only with standard reference genomes, and are best suited for small-scale projects. To address these limitations, we developed Crisflash, a software t...

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Detalles Bibliográficos
Autores principales: Jacquin, Adrien L S, Odom, Duncan T, Lukk, Margus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Applications Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735888/
https://www.ncbi.nlm.nih.gov/pubmed/30649181
http://dx.doi.org/10.1093/bioinformatics/btz019

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735888/
https://www.ncbi.nlm.nih.gov/pubmed/30649181
http://dx.doi.org/10.1093/bioinformatics/btz019

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