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Crisflash: open-source software to generate CRISPR guide RNAs against genomes annotated with individual variation
SUMMARY: CRISPR/Cas9 system requires short guide RNAs (sgRNAs) to direct genome modification. Most currently available tools for sgRNA design operate only with standard reference genomes, and are best suited for small-scale projects. To address these limitations, we developed Crisflash, a software t...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735888/ https://www.ncbi.nlm.nih.gov/pubmed/30649181 http://dx.doi.org/10.1093/bioinformatics/btz019 |