Targeted DNA transposition in vitro using a dCas9-transposase fusion protein

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates efficient Cas9 targeting of the mariner transposon Hsma...

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Detalles Bibliográficos
Autores principales: Bhatt, Shivam, Chalmers, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735945/
https://www.ncbi.nlm.nih.gov/pubmed/31429873
http://dx.doi.org/10.1093/nar/gkz552

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735945/
https://www.ncbi.nlm.nih.gov/pubmed/31429873
http://dx.doi.org/10.1093/nar/gkz552

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