Biological surface properties in extracellular vesicles and their effect on cargo proteins

Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol...

Descripción completa

Detalles Bibliográficos
Autores principales: Santucci, Laura, Bruschi, Maurizio, Del Zotto, Genny, Antonini, Francesca, Ghiggeri, Gian Marco, Panfoli, Isabella, Candiano, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736982/
https://www.ncbi.nlm.nih.gov/pubmed/31506490
http://dx.doi.org/10.1038/s41598-019-47598-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736982/
https://www.ncbi.nlm.nih.gov/pubmed/31506490
http://dx.doi.org/10.1038/s41598-019-47598-3

Ejemplares similares