Site-Directed Alkylation Detected by In-Gel Fluorescence (SDAF) to Determine the Topology Map and Probe the Solvent Accessibility of Membrane Proteins

The topology of helix-bundle membrane proteins provides low-resolution structural information with regard to the number and orientation of membrane-spanning helices, as well as the sidedness of intra/extra-cellular domains. In the past decades, several strategies have been developed to experimentall...

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Detalles Bibliográficos
Autores principales: Lin, Yu-Hung, Lin, Sung-Yao, Li, Guan-Syun, Weng, Shao-En, Tzeng, Shu-Ling, Hsiao, Yu-Hsuan, Hu, Nien-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739316/
https://www.ncbi.nlm.nih.gov/pubmed/31511541
http://dx.doi.org/10.1038/s41598-019-49292-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739316/
https://www.ncbi.nlm.nih.gov/pubmed/31511541
http://dx.doi.org/10.1038/s41598-019-49292-w

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