Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

BACKGROUND: Antibody fragments can be expressed in Escherichia coli, where they are commonly directed to the periplasm via Sec pathway to enable disulphide bridge formations and correct folding. In order to transport antibody fragments to the periplasmic space via Sec pathway, they are equipped with...

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Detalles Bibliográficos
Autores principales: Kulmala, Antti, Huovinen, Tuomas, Lamminmäki, Urpo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6745802/
https://www.ncbi.nlm.nih.gov/pubmed/31526395
http://dx.doi.org/10.1186/s12934-019-1210-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6745802/
https://www.ncbi.nlm.nih.gov/pubmed/31526395
http://dx.doi.org/10.1186/s12934-019-1210-1

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