Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells

Extracellular expression is essential for the function of secreted and cell surface proteins. Proper intracellular trafficking depends on protein interactions in multiple subcellular compartments. Co-immunoprecipitation and the yeast two-hybrid system are commonly used to investigate protein-protein...

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Detalles Bibliográficos
Autores principales: Wang, Hao, He, Meiling, Willard, Belinda, Wu, Qingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2019
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746336/
https://www.ncbi.nlm.nih.gov/pubmed/31528665
http://dx.doi.org/10.21769/BioProtoc.3258

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746336/
https://www.ncbi.nlm.nih.gov/pubmed/31528665
http://dx.doi.org/10.21769/BioProtoc.3258

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