Cargando…

Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells

Extracellular expression is essential for the function of secreted and cell surface proteins. Proper intracellular trafficking depends on protein interactions in multiple subcellular compartments. Co-immunoprecipitation and the yeast two-hybrid system are commonly used to investigate protein-protein...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Hao, He, Meiling, Willard, Belinda, Wu, Qingyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2019
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746336/ https://www.ncbi.nlm.nih.gov/pubmed/31528665 http://dx.doi.org/10.21769/BioProtoc.3258

Ejemplares similares

Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins
por: Rosenberg, Michael, et al.
Publicado: (2021)

Fast chromatin immunoprecipitation assay
por: Nelson, Joel D., et al.
Publicado: (2006)

Cell-free chromatin immunoprecipitation to detect molecular pathways in heart transplantation
por: Jang, Moon Kyoo, et al.
Publicado: (2023)

Optimized infrared photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (IR-PAR-CLIP) protocol identifies novel IGF2BP3-interacting RNAs in colon cancer cells
por: Anisimova, Aleksandra S., et al.
Publicado: (2023)

A plug and play microfluidic platform for standardized sensitive low-input chromatin immunoprecipitation
por: Dirks, René A.M., et al.
Publicado: (2021)

Quantitative proteomic analysis reveals concurrent RNA–protein interactions and identifies new RNA-binding proteins in Saccharomyces cerevisiae
por: Klass, Daniel M., et al.
Publicado: (2013)

Enhanced immunoprecipitation techniques for the identification of RNA-binding protein partners: IGF2BP1 interactions in mammary epithelial cells
por: Fakhraldeen, Saja A., et al.
Publicado: (2022)

Extensive chromatin fragmentation improves enrichment of protein binding sites in chromatin immunoprecipitation experiments
por: Fan, Xiaochun, et al.
Publicado: (2008)

Chipper: discovering transcription-factor targets from chromatin immunoprecipitation microarrays using variance stabilization
por: Gibbons, Francis D, et al.
Publicado: (2005)

TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay
por: Akhtar, Junaid, et al.
Publicado: (2019)

RNA-DNA hybrid (R-loop) immunoprecipitation mapping: an analytical workflow to evaluate inherent biases
por: Halász, László, et al.
Publicado: (2017)

Cell‐free chromatin immunoprecipitation can determine tumor gene expression in lung cancer patients
por: Trier Maansson, Christoffer, et al.
Publicado: (2023)

InSite: a computational method for identifying protein-protein interaction binding sites on a proteome-wide scale
por: Wang, Haidong, et al.
Publicado: (2007)

AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation
por: Aldridge, Sarah, et al.
Publicado: (2013)

Identification of Inflammatory Proteomics Networks of Toll-like Receptor 4 through Immunoprecipitation-Based Chemical Cross-Linking Proteomics
por: Shahinuzzaman, A. D. A., et al.
Publicado: (2022)

Optimization of experimental design parameters for high-throughput chromatin immunoprecipitation studies
por: Ponzielli, Romina, et al.
Publicado: (2008)

The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation
por: van Werven, Folkert J., et al.
Publicado: (2006)

Ritornello: high fidelity control-free chromatin immunoprecipitation peak calling
por: Stanton, Kelly P., et al.
Publicado: (2017)

Immunoprecipitation methods to identify S-glutathionylation in target proteins
por: Butturini, Elena, et al.
Publicado: (2019)

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
por: Emmott, Edward, et al.
Publicado: (2014)

A comparative analysis of genome-wide chromatin immunoprecipitation data for mammalian transcription factors
por: Ji, Hongkai, et al.
Publicado: (2006)

A simplified and sensitive immunoprecipitation approach for the analysis of HSF1 in murine liver tissue
por: Trivedi, Rachana, et al.
Publicado: (2021)

Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation Efficiency
por: Cacioppo, Roberta, et al.
Publicado: (2023)

PWAS: proteome-wide association study—linking genes and phenotypes by functional variation in proteins
por: Brandes, Nadav, et al.
Publicado: (2020)

Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA
por: Statham, Aaron L., et al.
Publicado: (2012)

Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: a versatile and rapid ChIP procedure
por: Harmeyer, Kayla M., et al.
Publicado: (2015)

Binding of lac repressor-GFP fusion protein to lac operator sites inserted in the tobacco chloroplast genome examined by chromatin immunoprecipitation
por: Newell, Christine A., et al.
Publicado: (2010)

Quick-irCLIP: Interrogating protein-RNA interactions using a rapid and simple cross-linking and immunoprecipitation technique
por: Kaczynski, Tadeusz, et al.
Publicado: (2019)

Systematic evaluation of chromatin immunoprecipitation sequencing to study histone occupancy in dormancy transitions of grapevine buds
por: Hermawaty, Dina, et al.
Publicado: (2023)

EGFR transcription in non‐small‐cell lung cancer tumours can be revealed in ctDNA by cell‐free chromatin immunoprecipitation (cfChIP)
por: Månsson, Christoffer Trier, et al.
Publicado: (2021)

Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
por: Kuzembayeva, Malika, et al.
Publicado: (2012)

Protease‐resistant streptavidin for interaction proteomics
por: Rafiee, Mahmoud‐Reza, et al.
Publicado: (2020)

Visualizing the Ensemble Structures of Protein Complexes Using Chemical Cross-Linking Coupled with Mass Spectrometry
por: Gong, Zhou, et al.
Publicado: (2015)

Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions
por: Burckhardt, Christoph J., et al.
Publicado: (2021)

μChIP—a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsies
por: Dahl, John Arne, et al.
Publicado: (2008)

Inferring protein–protein interaction complexes from immunoprecipitation data
por: Kutzera, Joachim, et al.
Publicado: (2013)

Nanobody®-based chromatin immunoprecipitation/micro-array analysis for genome-wide identification of transcription factor DNA binding sites
por: Nguyen-Duc, Trong, et al.
Publicado: (2013)

Directed shotgun proteomics guided by saturated RNA-seq identifies a complete expressed prokaryotic proteome
por: Omasits, Ulrich, et al.
Publicado: (2013)

Proteome‐wide analysis of phospho‐regulated PDZ domain interactions
por: Sundell, Gustav N, et al.
Publicado: (2018)

Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase
por: Peace, Jared M., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_lju0ua7f0ugo60n1dakq9sf1fb