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GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve
GPR40/FFAR1 is a Gq protein-coupled receptor expressed in pancreatic β cells and enteroendocrine cells, and mediates insulin and incretin secretion to regulate feeding behavior. Several GPR40 full agonists have been reported to reduce food intake in rodents by regulating gut hormone secretion in add...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746387/ https://www.ncbi.nlm.nih.gov/pubmed/31525244 http://dx.doi.org/10.1371/journal.pone.0222653 |
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author | Ueno, Hikaru Ito, Ryo Abe, Shin-ichi Ogino, Hitomi Maruyama, Minoru Miyashita, Hirohisa Miyamoto, Yasufumi Moritoh, Yusuke Tsujihata, Yoshiyuki Takeuchi, Koji Nishigaki, Nobuhiro |
author_facet | Ueno, Hikaru Ito, Ryo Abe, Shin-ichi Ogino, Hitomi Maruyama, Minoru Miyashita, Hirohisa Miyamoto, Yasufumi Moritoh, Yusuke Tsujihata, Yoshiyuki Takeuchi, Koji Nishigaki, Nobuhiro |
author_sort | Ueno, Hikaru |
collection | PubMed |
description | GPR40/FFAR1 is a Gq protein-coupled receptor expressed in pancreatic β cells and enteroendocrine cells, and mediates insulin and incretin secretion to regulate feeding behavior. Several GPR40 full agonists have been reported to reduce food intake in rodents by regulating gut hormone secretion in addition to their potent glucose-lowering effects; however, detailed mechanisms of feeding suppression are still unknown. In the present study, we characterized T-3601386, a novel compound with potent full agonistic activity for GPR40, by using in vitro Ca(2+) mobilization assay in Chinese hamster ovary (CHO) cells expressing FFAR1 and in vivo hormone secretion assay. We also evaluated feeding suppression and weight loss after the administration of T-3601386 and investigated the involvement of the vagal nerve in these effects. T-3601386, but not a partial agonist fasiglifam, increased intracellular Ca(2+) levels in CHO cells with low FFAR1 expression, and single dosing of T-3601386 in diet-induced obese (DIO) rats elevated plasma incretin levels, suggesting full agonistic properties of T-3601386 against GPR40. Multiple doses of T-3601386, but not fasiglifam, in DIO rats showed dose-dependent weight loss accompanied by feeding suppression and durable glucagon-like peptide-1 elevation, all of which were completely abolished in Ffar1(-/-) mice. Immunohistochemical analysis in the nuclei of the solitary tract demonstrated that T-3601386 increased the number of c-Fos positive cells, which also disappeared in Ffar1(-/-) mice. Surgical vagotomy and drug-induced deafferentation counteracted the feeding suppression and weight loss induced by the administration of T-3601386. These results suggest that T-3601386 exerts incretin release and weight loss in a GPR40-dependent manner, and that afferent vagal nerves are important for the feeding suppression induced by GPR40 full agonism. Our novel findings raise the possibility that GPR40 full agonist can induce periphery-derived weight reduction, which may provide benefits such as less adverse effects in central nervous system compared to centrally-acting anti-obesity drugs. |
format | Online Article Text |
id | pubmed-6746387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67463872019-09-27 GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve Ueno, Hikaru Ito, Ryo Abe, Shin-ichi Ogino, Hitomi Maruyama, Minoru Miyashita, Hirohisa Miyamoto, Yasufumi Moritoh, Yusuke Tsujihata, Yoshiyuki Takeuchi, Koji Nishigaki, Nobuhiro PLoS One Research Article GPR40/FFAR1 is a Gq protein-coupled receptor expressed in pancreatic β cells and enteroendocrine cells, and mediates insulin and incretin secretion to regulate feeding behavior. Several GPR40 full agonists have been reported to reduce food intake in rodents by regulating gut hormone secretion in addition to their potent glucose-lowering effects; however, detailed mechanisms of feeding suppression are still unknown. In the present study, we characterized T-3601386, a novel compound with potent full agonistic activity for GPR40, by using in vitro Ca(2+) mobilization assay in Chinese hamster ovary (CHO) cells expressing FFAR1 and in vivo hormone secretion assay. We also evaluated feeding suppression and weight loss after the administration of T-3601386 and investigated the involvement of the vagal nerve in these effects. T-3601386, but not a partial agonist fasiglifam, increased intracellular Ca(2+) levels in CHO cells with low FFAR1 expression, and single dosing of T-3601386 in diet-induced obese (DIO) rats elevated plasma incretin levels, suggesting full agonistic properties of T-3601386 against GPR40. Multiple doses of T-3601386, but not fasiglifam, in DIO rats showed dose-dependent weight loss accompanied by feeding suppression and durable glucagon-like peptide-1 elevation, all of which were completely abolished in Ffar1(-/-) mice. Immunohistochemical analysis in the nuclei of the solitary tract demonstrated that T-3601386 increased the number of c-Fos positive cells, which also disappeared in Ffar1(-/-) mice. Surgical vagotomy and drug-induced deafferentation counteracted the feeding suppression and weight loss induced by the administration of T-3601386. These results suggest that T-3601386 exerts incretin release and weight loss in a GPR40-dependent manner, and that afferent vagal nerves are important for the feeding suppression induced by GPR40 full agonism. Our novel findings raise the possibility that GPR40 full agonist can induce periphery-derived weight reduction, which may provide benefits such as less adverse effects in central nervous system compared to centrally-acting anti-obesity drugs. Public Library of Science 2019-09-16 /pmc/articles/PMC6746387/ /pubmed/31525244 http://dx.doi.org/10.1371/journal.pone.0222653 Text en © 2019 Ueno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ueno, Hikaru Ito, Ryo Abe, Shin-ichi Ogino, Hitomi Maruyama, Minoru Miyashita, Hirohisa Miyamoto, Yasufumi Moritoh, Yusuke Tsujihata, Yoshiyuki Takeuchi, Koji Nishigaki, Nobuhiro GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title | GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title_full | GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title_fullStr | GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title_full_unstemmed | GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title_short | GPR40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
title_sort | gpr40 full agonism exerts feeding suppression and weight loss through afferent vagal nerve |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746387/ https://www.ncbi.nlm.nih.gov/pubmed/31525244 http://dx.doi.org/10.1371/journal.pone.0222653 |
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