A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression

The Cre/loxP recombination system has revolutionized the ability to genetically manipulate animal genomes in order to conditionally control gene expression. With recent advances in genome editing, barriers to manipulating the rat genome have been overcome and it is now possible to generate new rat s...

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Autores principales: Bryda, Elizabeth C., Men, Hongsheng, Davis, Daniel J., Bock, Anagha S., Shaw, Mary L., Chesney, Kari L., Hankins, Miriam A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746712/
https://www.ncbi.nlm.nih.gov/pubmed/31527612
http://dx.doi.org/10.1038/s41598-019-49783-w
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author Bryda, Elizabeth C.
Men, Hongsheng
Davis, Daniel J.
Bock, Anagha S.
Shaw, Mary L.
Chesney, Kari L.
Hankins, Miriam A.
author_facet Bryda, Elizabeth C.
Men, Hongsheng
Davis, Daniel J.
Bock, Anagha S.
Shaw, Mary L.
Chesney, Kari L.
Hankins, Miriam A.
author_sort Bryda, Elizabeth C.
collection PubMed
description The Cre/loxP recombination system has revolutionized the ability to genetically manipulate animal genomes in order to conditionally control gene expression. With recent advances in genome editing, barriers to manipulating the rat genome have been overcome and it is now possible to generate new rat strains (Cre drivers) in which Cre recombinase expression is carefully controlled temporally and/or spatially. However, the ability to evaluate and characterize these Cre driver strains is limited by the availability of reliable reporter rat strains. Here, we describe the generation and characterization of a new transgenic rat strain in which conditional expression of the ZsGreen fluorescent protein gene requires the presence of exogenous Cre recombinase. Breeding Cre-expressing rat strains to this stable ZsGreen reporter strain provides an ideal method for validating new rat Cre driver lines and will greatly accelerate the characterization pipeline.
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spelling pubmed-67467122019-09-27 A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression Bryda, Elizabeth C. Men, Hongsheng Davis, Daniel J. Bock, Anagha S. Shaw, Mary L. Chesney, Kari L. Hankins, Miriam A. Sci Rep Article The Cre/loxP recombination system has revolutionized the ability to genetically manipulate animal genomes in order to conditionally control gene expression. With recent advances in genome editing, barriers to manipulating the rat genome have been overcome and it is now possible to generate new rat strains (Cre drivers) in which Cre recombinase expression is carefully controlled temporally and/or spatially. However, the ability to evaluate and characterize these Cre driver strains is limited by the availability of reliable reporter rat strains. Here, we describe the generation and characterization of a new transgenic rat strain in which conditional expression of the ZsGreen fluorescent protein gene requires the presence of exogenous Cre recombinase. Breeding Cre-expressing rat strains to this stable ZsGreen reporter strain provides an ideal method for validating new rat Cre driver lines and will greatly accelerate the characterization pipeline. Nature Publishing Group UK 2019-09-16 /pmc/articles/PMC6746712/ /pubmed/31527612 http://dx.doi.org/10.1038/s41598-019-49783-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bryda, Elizabeth C.
Men, Hongsheng
Davis, Daniel J.
Bock, Anagha S.
Shaw, Mary L.
Chesney, Kari L.
Hankins, Miriam A.
A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title_full A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title_fullStr A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title_full_unstemmed A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title_short A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
title_sort novel conditional zsgreen-expressing transgenic reporter rat strain for validating cre recombinase expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746712/
https://www.ncbi.nlm.nih.gov/pubmed/31527612
http://dx.doi.org/10.1038/s41598-019-49783-w
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