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Active Site Architecture and Reaction Mechanism Determination of Cold Adapted β-d-galactosidase from Arthrobacter sp. 32cB

ArthβDG is a dimeric, cold-adapted β-d-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthβDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthβDG_E441Q...

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Detalles Bibliográficos
Autores principales: Rutkiewicz, Maria, Bujacz, Anna, Wanarska, Marta, Wierzbicka-Wos, Anna, Cieslinski, Hubert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747455/
https://www.ncbi.nlm.nih.gov/pubmed/31484304
http://dx.doi.org/10.3390/ijms20174301