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Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume

To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of Gastrodia elata (G. elata), a fresh G. elata sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used...

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Autores principales: Li, Yun, Liu, Xiao-Qian, Liu, Shan-Shan, Liu, Da-hui, Wang, Xiao, Wang, Zhi-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749462/
https://www.ncbi.nlm.nih.gov/pubmed/31480235
http://dx.doi.org/10.3390/molecules24173159
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author Li, Yun
Liu, Xiao-Qian
Liu, Shan-Shan
Liu, Da-hui
Wang, Xiao
Wang, Zhi-Min
author_facet Li, Yun
Liu, Xiao-Qian
Liu, Shan-Shan
Liu, Da-hui
Wang, Xiao
Wang, Zhi-Min
author_sort Li, Yun
collection PubMed
description To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of Gastrodia elata (G. elata), a fresh G. elata sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composition in steamed and fresh G. elata. Finally, 25 components were identified in G. elata based on the characteristic fragments of the compounds and the changes of the 25 components of fresh and steamed G. elata were compared by the relative content. Hydrolysis experiments and enzymatic hydrolysis experiments of 10 monomer compounds simulating the G. elata steaming process were carried out for the first time. As a result, hydrolysis experiments proved that free gastrodin or p-hydroxybenzyl alcohol could be obtained by breaking ester bond or ether bond during the steaming process of G. elata. Enzymatic experiments showed that steaming played an important role in the protection of gastrodin, confirming the hypothesis that steaming can promote the conversion of chemical constituents of G. elata—inhibiting enzymatic degradation. This experiment clarified the scientific mechanism of the traditional steaming method of G. elata and provided reference for how to apply G. elata decoction to some extent.
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spelling pubmed-67494622019-09-27 Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume Li, Yun Liu, Xiao-Qian Liu, Shan-Shan Liu, Da-hui Wang, Xiao Wang, Zhi-Min Molecules Article To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of Gastrodia elata (G. elata), a fresh G. elata sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composition in steamed and fresh G. elata. Finally, 25 components were identified in G. elata based on the characteristic fragments of the compounds and the changes of the 25 components of fresh and steamed G. elata were compared by the relative content. Hydrolysis experiments and enzymatic hydrolysis experiments of 10 monomer compounds simulating the G. elata steaming process were carried out for the first time. As a result, hydrolysis experiments proved that free gastrodin or p-hydroxybenzyl alcohol could be obtained by breaking ester bond or ether bond during the steaming process of G. elata. Enzymatic experiments showed that steaming played an important role in the protection of gastrodin, confirming the hypothesis that steaming can promote the conversion of chemical constituents of G. elata—inhibiting enzymatic degradation. This experiment clarified the scientific mechanism of the traditional steaming method of G. elata and provided reference for how to apply G. elata decoction to some extent. MDPI 2019-08-30 /pmc/articles/PMC6749462/ /pubmed/31480235 http://dx.doi.org/10.3390/molecules24173159 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yun
Liu, Xiao-Qian
Liu, Shan-Shan
Liu, Da-hui
Wang, Xiao
Wang, Zhi-Min
Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title_full Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title_fullStr Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title_full_unstemmed Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title_short Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume
title_sort transformation mechanisms of chemical ingredients in steaming process of gastrodia elata blume
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749462/
https://www.ncbi.nlm.nih.gov/pubmed/31480235
http://dx.doi.org/10.3390/molecules24173159
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