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Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells

Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma(null) (NSG) mice engrafted with human cord blood (hCB)-CD34(+) cells have been proposed to be a valuable tool to reproduce human immune...

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Autores principales: Tanaskovic, Olga, Verga Falzacappa, Maria Vittoria, Pelicci, Pier Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752751/
https://www.ncbi.nlm.nih.gov/pubmed/31536492
http://dx.doi.org/10.1371/journal.pone.0217345
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author Tanaskovic, Olga
Verga Falzacappa, Maria Vittoria
Pelicci, Pier Giuseppe
author_facet Tanaskovic, Olga
Verga Falzacappa, Maria Vittoria
Pelicci, Pier Giuseppe
author_sort Tanaskovic, Olga
collection PubMed
description Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma(null) (NSG) mice engrafted with human cord blood (hCB)-CD34(+) cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34(+) cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34(+) NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34(+) NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34(+) NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells.
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spelling pubmed-67527512019-09-27 Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells Tanaskovic, Olga Verga Falzacappa, Maria Vittoria Pelicci, Pier Giuseppe PLoS One Research Article Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma(null) (NSG) mice engrafted with human cord blood (hCB)-CD34(+) cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34(+) cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34(+) NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34(+) NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34(+) NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells. Public Library of Science 2019-09-19 /pmc/articles/PMC6752751/ /pubmed/31536492 http://dx.doi.org/10.1371/journal.pone.0217345 Text en © 2019 Tanaskovic et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tanaskovic, Olga
Verga Falzacappa, Maria Vittoria
Pelicci, Pier Giuseppe
Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title_full Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title_fullStr Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title_full_unstemmed Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title_short Human cord blood (hCB)-CD34(+) humanized mice fail to reject human acute myeloid leukemia cells
title_sort human cord blood (hcb)-cd34(+) humanized mice fail to reject human acute myeloid leukemia cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752751/
https://www.ncbi.nlm.nih.gov/pubmed/31536492
http://dx.doi.org/10.1371/journal.pone.0217345
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