Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing

Transient expression of the CRISPR/Cas9 machinery will not only reduce risks of mutagenesis from off-target activities, but also decrease possible immune response to Cas9 protein. Building on our recent developing of a system able to package up to 100 copies of Cas9 mRNA in each lentivirus-like part...

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Detalles Bibliográficos
Autores principales: Lyu, Pin, Javidi-Parsijani, Parisa, Atala, Anthony, Lu, Baisong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753487/
https://www.ncbi.nlm.nih.gov/pubmed/31299082
http://dx.doi.org/10.1093/nar/gkz605

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753487/
https://www.ncbi.nlm.nih.gov/pubmed/31299082
http://dx.doi.org/10.1093/nar/gkz605

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