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Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

BACKGROUND: CRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are time-consuming and can be of mutagenic nature. These developments allow the study of large gene families that contain putatively redun...

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Detalles Bibliográficos
Autores principales:	van Leeuwe, Tim M., Arentshorst, Mark, Ernst, Tim, Alazi, Ebru, Punt, Peter J., Ram, Arthur F. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754632/ https://www.ncbi.nlm.nih.gov/pubmed/31559019 http://dx.doi.org/10.1186/s40694-019-0076-7

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