Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells

[Image: see text] As an important post-transcriptional regulatory machinery mediated by ∼21nt short-interfering double-stranded RNA (siRNA), RNA interference (RNAi) is a powerful tool to delineate gene functions and develop therapeutics. However, effective RNAi-mediated silencing requires multiple s...

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Detalles Bibliográficos
Autores principales: Wang, Xi, Yuan, Chengfu, Huang, Bo, Fan, Jiaming, Feng, Yixiao, Li, Alexander J., Zhang, Bo, Lei, Yan, Ye, Zhenyu, Zhao, Ling, Cao, Daigui, Yang, Lijuan, Wu, Di, Chen, Xian, Liu, Bin, Wagstaff, William, He, Fang, Wu, Xiaoxing, Luo, Huaxiu, Zhang, Jing, Zhang, Meng, Haydon, Rex C., Luu, Hue H., Lee, Michael J., Moriatis Wolf, Jennifer, Huang, Ailong, He, Tong-Chuan, Zeng, Zongyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760290/
https://www.ncbi.nlm.nih.gov/pubmed/31465214
http://dx.doi.org/10.1021/acssynbio.9b00203

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760290/
https://www.ncbi.nlm.nih.gov/pubmed/31465214
http://dx.doi.org/10.1021/acssynbio.9b00203

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