Bioorthogonal protein-DNA conjugation methods for force spectroscopy

Accurate and stable site-specific attachment of DNA molecules to proteins is a requirement for many single-molecule force spectroscopy techniques. The most commonly used method still relies on maleimide chemistry involving cysteine residues in the protein of interest. Studies have consequently often...

Descripción completa

Detalles Bibliográficos
Autores principales: Synakewicz, Marie, Bauer, Daniela, Rief, Matthias, Itzhaki, Laura S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761116/
https://www.ncbi.nlm.nih.gov/pubmed/31554828
http://dx.doi.org/10.1038/s41598-019-49843-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761116/
https://www.ncbi.nlm.nih.gov/pubmed/31554828
http://dx.doi.org/10.1038/s41598-019-49843-1

Ejemplares similares