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Conformation and mechanical property of rpoS mRNA inhibitory stem studied by optical tweezers and X-ray scattering

3′ downstream inhibitory stem plays a crucial role in locking rpoS mRNA 5' untranslated region in a self-inhibitory state. Here, we used optical tweezers to study the unfolding/refolding of rpoS inhibitory stem in the absence and presence of Mg(2+). We found adding Mg(2+) decreased the free ene...

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Detalles Bibliográficos
Autores principales: Hu, Xinyao, Li, Xuanling, Yang, Lingna, Zhu, Yilin, Shi, Yunyu, Li, Yinmei, Wang, Haowei, Gong, Qingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762075/
https://www.ncbi.nlm.nih.gov/pubmed/31557220
http://dx.doi.org/10.1371/journal.pone.0222938